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Brillouin microscopy is an emerging tool of biomedical imaging [1]. While for a long time Brillouin spectroscopy was considered an exquisite tool of precise spectroscopy, recent advancements in instrumentation, methods of signal and data analysis, and multimodal imaging have paved the way to new applications in the broad area of biomedical research.
In my presentation, I will review the recent advances of instrumentation, which result in much higher speed of Brillouin spectroscopy and imaging, while providing better discrimination against the background scattering and better sensitivity of Brillouin microscopy measurements. I will also discuss some new developments which can potentially lead to improved spatial resolution of Brillouin microscopy imaging and which can utilize the quantum nature of light to improve the signal discrimination.
The above improvements in instrumentation translated into a number of applications, which allowed tissue assessment using Brillouin microscopy (see, for example, [2]) to be an essential component of broader medical protocols [3-4] to evaluate undergoing modifications of tissues in response to a certain treatment procedure. I will attempt to make sense out of such unusual sensitivity of Brillouin microscopy to the physiologically significant variations in tissue properties and will outline other potential directions of biological and biomedical research, which can be facilitated by continuously improving Brillouin microscopy instruments.
[1] Z Meng, AJ Traverso, CW Ballmann, MA Troyanova-Wood, VV Yakovlev, Advances in Optics and Photonics 8 (2), 300-327 (2016).
[2] M Troyanova‐Wood, C Gobbell, Z Meng, AA Gashev, VV Yakovlev, Journal of Biophotonics 10(12), 1694-1702 (2017).
[3] OY Gasheva, IT Nizamutdinova, L Hargrove, C Gobbell, M Troyanova-Wood, SF Alpini, S Pal, C Du, AR Hitt, VV Yakovlev, MK Newell-Rogers, DC Zawieja, CJ Meininger, GD Alpini, H Francis, AA Gashev, Journal of Experimental Medicine (2018), Under review.
[4] D Akilbekova, B Ogay, T Yakupov, M Saesenova, B Umbayev, VV Yakovlev, A Nurakhmetov, K Tazhin, and ZN Utegulov, Journal of Biomedical Optics (2018), Under revisions.
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