Quantitative phase imaging of adherent mammalian cells: a comparison of three different techniques (Conference Presentation)

Cédric Allier; Julien Savatier; Serge Monneret; Yves Usson; Lionel Hervé; Ondrej Mandula; Pierre Blandin; Sophie Morales

doi:10.1117/12.2508421

4 March 2019 Quantitative phase imaging of adherent mammalian cells: a comparison of three different techniques (Conference Presentation)

Cédric Allier, Julien Savatier, Serge Monneret, Yves Usson, Lionel Hervé, Ondrej Mandula, Pierre Blandin, Sophie Morales

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 108870C (2019) https://doi.org/10.1117/12.2508421
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Quantitative phase imaging (QPI) allows the monitoring of adherent cell cultures continuously over long time periods and it delivers an image of the cell with pixel intensities corresponding to the optical path difference (OPD). These images can be processed to quantify several cellular features. In particular, cell OPD measurements allows the estimation of the cell dry mass, an important metric to study cell mass and growth kinetics. If the ability of QPI to provide phase-contrast images of cells is taken for granted, the accuracy and the precision of QPI cell OPD measurements can still be questioned. Indeed, the reported QPI cell measurements have not yet been assessed with any reference method (e.g. microfluidic resonators). And there is a lack of independent experimental comparison and validation which can hinder the acceptance of QPI in the realms of live-cell mass profiling. With the aim of filling this gap, here we compare three different methods: digital holographic microscopy, quadriwave lateral sheering interferometry and lens-free microscopy (not yet established as a QPI technique). The experimental design is based on the inter-modality comparisons of OPD measurements performed over several tens of cells. To ensure consistency, we performed OPD measurements on a fixed cell culture the same day on the same location. Importantly, the statistical analysis of these measurements allowed us to estimate the precision of QPI cell OPD measurements without any reference material. In addition, we have evaluated the influence of the post-processing steps (baseline subtraction, cell segmentation) on the precision of QPI cell measurements.

Conference Presentation

Citation Download Citation

Cédric Allier, Julien Savatier, Serge Monneret, Yves Usson, Lionel Hervé, Ondrej Mandula, Pierre Blandin, and Sophie Morales "Quantitative phase imaging of adherent mammalian cells: a comparison of three different techniques (Conference Presentation)", Proc. SPIE 10887, Quantitative Phase Imaging V, 108870C (4 March 2019); https://doi.org/10.1117/12.2508421

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Phase imaging

Digital holography

Image processing

Microscopy

Statistical analysis

Holographic interferometry

Holography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years