Fourier-filtering based size-encoded images for label-free tracking of sub-cellular organelles in neurons

Mohammad Naser; Erin Kelly; Corey Ditmars; Rene S. Schloss; Bonnie L. Firestein; Nada N. Boustany

doi:10.1117/12.2510138

4 March 2019 Fourier-filtering based size-encoded images for label-free tracking of sub-cellular organelles in neurons

Mohammad Naser, Erin Kelly, Corey Ditmars, Rene S. Schloss, Bonnie L. Firestein, Nada N. Boustany

Proceedings Volume 10888, Biophysics, Biology and Biophotonics IV: the Crossroads; 108880H (2019) https://doi.org/10.1117/12.2510138
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Mitochondrial dynamics such as fission, fusion and movement can reveal the physiological status of neurons and, most importantly, serve as diagnostic measures for several neurodegenerative diseases. Traditionally fluorescent probes have been used to track mitochondrial dynamics in neurons. However, neurons show low transfection efficiency, presenting challenges for the use of genetically-encoded fluorescent markers. Alternatively, synthetic fluorescent dyes are shown to hinder mitochondrial motility. In addition, all types of fluorescent probes are subject to photo-bleaching which precludes imaging for longer periods of time. To circumvent these issues, we propose a light-scattering based label-free technique called Optical Scatter Imaging (OSI) that is sensitive to changes in morphology. In this work, we employ a previously reported label-free parameter to probe the change in the size of organelles such as mitochondria as they undergo fusion or fission in neurons. In addition, we present a technique to track organelle motion using kymographs obtained from the label-free images and compare them with those obtained from fluorescent images. We demonstrate that the label-free kymograph can track organelles such as mitochondria even after the sample is photo-bleached.

Conference Presentation

Citation Download Citation

Mohammad Naser, Erin Kelly, Corey Ditmars, Rene S. Schloss, Bonnie L. Firestein, and Nada N. Boustany "Fourier-filtering based size-encoded images for label-free tracking of sub-cellular organelles in neurons", Proc. SPIE 10888, Biophysics, Biology and Biophotonics IV: the Crossroads, 108880H (4 March 2019); https://doi.org/10.1117/12.2510138

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available