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13 March 2019 Temporal multiplexing FRET: using single Tb-to-quantum dot pair distance-tuning (Conference Presentation)
Jiajia Guo, Xue Qiu, Carlos Mingoes, Jeffrey Deschamps, Igor Medintz, Niko Hildebrandt
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Proceedings Volume 10892, Colloidal Nanoparticles for Biomedical Applications XIV; 108920O (2019) https://doi.org/10.1117/12.2512220
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
In this work, we utilized a unique polyhistidine peptide-DNA to conjugate with DHLA-capped QD625 (QD625) and different lengths of ssDNAs which were complementary to different parts of polyhistidine peptide-DNA to conjugate with Tb, and therefore, Tb are located away from the surface of QD with the length of polyhistidine peptide in addition of the length of ssDNA of 0, 2, 4, 6, 10 and 14 bases, or the lengths of dsDNA of 10, 14, 18, 22 and 26 base pairs, respectively. The lifetime of QD became longer and longer as Tb was moving away from QD. The distances calculated from Tb and QD channels by fitting were in an excellent agreement with the model that demonstrated temporal multiplexing FRET using a single Tb-QD FRET pair is successfully developed and can be used as biosensor.
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Jiajia Guo, Xue Qiu, Carlos Mingoes, Jeffrey Deschamps, Igor Medintz, and Niko Hildebrandt "Temporal multiplexing FRET: using single Tb-to-quantum dot pair distance-tuning (Conference Presentation)", Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920O (13 March 2019); https://doi.org/10.1117/12.2512220
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KEYWORDS
Fluorescence resonance energy transfer
Multiplexing
Terbium
Biosensors
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