A fast and reproducible ELISA laser platform

Xiaotian Tan; Qiushu Chen; Hongbo Zhu; Yuan Gong; Yu-Cheng Chen; Xuzhou Li; Xiaoqin Wu; Maxwell Wei-Hao Li; Wenyi Liu; Xudong Fan

doi:10.1117/12.2507517

7 March 2019 A fast and reproducible ELISA laser platform

Xiaotian Tan, Qiushu Chen, Hongbo Zhu, Yuan Gong, Yu-Cheng Chen, Xuzhou Li, Xiaoqin Wu, Maxwell Wei-Hao Li, Wenyi Liu, Xudong Fan

Author Affiliations +

Proceedings Volume 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI; 108950V (2019) https://doi.org/10.1117/12.2507517
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optofluidic bio-lasers are currently of high interest for sensitive, intra-cavity, biochemical analysis. In comparison with conventional methods such as fluorescence and colorimetric detection, lasers provide us with a method for amplifying small concentration differences in the gain medium, thus achieving high sensitivity. Our previous research has demonstrated that sandwich IL-6 ELISA performed in capillary-based optofluidic laser cavity was able to achieve ultrahigh detection sensitivity (LOD between 1-10 fg/ml) with a small sample volume (~20 μL). However, such approach has several limitations such as low repeatability and long assay time (~8 hours in total, 7 hours for laser measurements). Here, we developed a novel on-chip ELISA laser platform by directly fabricating micro-wells on dielectric mirrors for immunosorbent reactions. Polystyrene microbeads of 30 μm in diameter were placed in the wells to optically enhance the resonance cavity during laser measurement, thus significantly improving reliability, shortening assay time (~1.5 hours, 30 minutes for laser measurements) while maintaining the attractive features such as small sample volume and very high sensitivity (LOD ~0.1 pg/mL for IL-6). This work pushes the ELISA laser one step closer to solving problems in realworld biochemical analysis.

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Xiaotian Tan, Qiushu Chen, Hongbo Zhu, Yuan Gong, Yu-Cheng Chen, Xuzhou Li, Xiaoqin Wu, Maxwell Wei-Hao Li, Wenyi Liu, and Xudong Fan "A fast and reproducible ELISA laser platform", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 108950V (7 March 2019); https://doi.org/10.1117/12.2507517

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