Detection of blood microcirculation of chicken embryo using LASCA-imaging with application of the new techniques of optical clearing

O. V. Ulianova; A. A. Kolosova; N. N. Filonova; A. M. Lyapina; Yu. V. Saltykov; I. A. Subbotina; S. S. Zaytsev; I. G. Grashkina; S. S. Ulyanov; V. A. Feodorova

doi:10.1117/12.2523025

3 June 2019 Detection of blood microcirculation of chicken embryo using LASCA-imaging with application of the new techniques of optical clearing

O. V. Ulianova, A. A. Kolosova, N. N. Filonova, A. M. Lyapina, Yu. V. Saltykov, I. A. Subbotina, S. S. Zaytsev, I. G. Grashkina, S. S. Ulyanov, V. A. Feodorova

Author Affiliations +

Proceedings Volume 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine; 110650N (2019) https://doi.org/10.1117/12.2523025
Event: International Symposium on Optics and Biophotonics VI: Saratov Fall Meeting 2018, 2018, Saratov, Russian Federation

Abstract

Methods of t-LASCA and s-LASCA imaging have been adapted to the problem of monitoring of blood microcirculation in naïve chicken embryo. Set-up for LASCA imaging of the model chicken embryo is mounted. The novel original technique of preparing of optical windows for observation of a naïve chicken embryo is suggested. This technique is based on a removal of part of the natural egg shell using a citric acid solution with further optical clearing using glycerol and glucose.

Citation Download Citation

O. V. Ulianova, A. A. Kolosova, N. N. Filonova, A. M. Lyapina, Yu. V. Saltykov, I. A. Subbotina, S. S. Zaytsev, I. G. Grashkina, S. S. Ulyanov, and V. A. Feodorova "Detection of blood microcirculation of chicken embryo using LASCA-imaging with application of the new techniques of optical clearing ", Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650N (3 June 2019); https://doi.org/10.1117/12.2523025

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available