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3 June 2019 Amplification of output signal of laser scanning speckle-microscope using gold nanoparticles for detection of Chlamydia trachomatis bacteria. Part 1: theoretical study
O. V. Ulianova, N. N. Filonova, I. A. Subbotina, L. A. Dykman, S. S. Ulyanov, S. S. Zaytsev, Yu. V. Saltykov, A. M. Lyapina, I. G. Grashkina, O. S. Larionova, V. A. Feodorova
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Proceedings Volume 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine; 110650O (2019) https://doi.org/10.1117/12.2523029
Event: International Symposium on Optics and Biophotonics VI: Saratov Fall Meeting 2018, 2018, Saratov, Russian Federation
Abstract
Method of speckle-microscopy has been adapted to the problem of detection of Chlamydia trachomatis microbial cells in aqueous suspension and fixed on the glass. Combined system “C. trachomatis bacterial cell”-“monoclonal antibody”- “gold nanoparticle” has been used as a complicated scattering element in the case of formation of biospeckles with a small number of scatterers. Optical model of diffraction of strongly focused Gaussian beam on combined scattering system, containing gold nanoparticles, has been suggested. Simulation of speckles, forming with a small number of scattering nanoparticles, has been carried out. It has been demonstrated that the usage of nanoparticles allows to enhance essentially the output signal of speckle-microscope.
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O. V. Ulianova, N. N. Filonova, I. A. Subbotina, L. A. Dykman, S. S. Ulyanov, S. S. Zaytsev, Yu. V. Saltykov, A. M. Lyapina, I. G. Grashkina, O. S. Larionova, and V. A. Feodorova "Amplification of output signal of laser scanning speckle-microscope using gold nanoparticles for detection of Chlamydia trachomatis bacteria. Part 1: theoretical study", Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650O (3 June 2019); https://doi.org/10.1117/12.2523029
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KEYWORDS
Nanoparticles
Scattering
Gold
Laser scattering
Bacteria
Light scattering
Speckle
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