Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets

Pietro Strobbia; Yang Ran; Bridget M. Crawford; Vanessa Cupil-Garcia; Rodolfo Zentella; Hsin-Neng Wang; Tai-ping Sun; Tuan Vo-Dinh

doi:10.1117/12.2529844

9 September 2019 Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets

Pietro Strobbia, Yang Ran, Bridget M. Crawford, Vanessa Cupil-Garcia, Rodolfo Zentella, Hsin-Neng Wang, Tai-ping Sun, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII; 110822L (2019) https://doi.org/10.1117/12.2529844
Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States

Abstract

Molecular analysis has revolutionized many applications, including bio-safety, bio-engineering and biofuel research; however, there are limited practical tools for in situ detection during field work. New technology is needed to translate molecular advances from laboratory settings into the practical realm. The unique characteristics of plasmonic nanosensors have made them ideal candidates for field-ready sensing applications. Herein, we discuss the development of a fiber-based plasmonic sensor capable of direct detection (i.e., no washing steps required) of miRNA targets, which are detected by immerging the sensor in the sample solution. This sensor is composed of an optical fiber that is decorated with plasmonic nanoprobes based on silver-coated gold nanostars to detect target nucleic acids using the surface-enhanced Raman scattering sensing mechanism of nanoprobes referred to as inverse molecular sentinels. The fiber sensors were tested in extracts from leaves of plants that were induced to have different miRNA expression levels. The results indicate that the fiber sensors developed have the potential to be a powerful tool for field analysis.

Citation Download Citation

Pietro Strobbia, Yang Ran, Bridget M. Crawford, Vanessa Cupil-Garcia, Rodolfo Zentella, Hsin-Neng Wang, Tai-ping Sun, and Tuan Vo-Dinh "Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets", Proc. SPIE 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII, 110822L (9 September 2019); https://doi.org/10.1117/12.2529844

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