Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit

Meiai Lin; Shuyu Zhang; Qiao Liu; Changshun Shao; Xuantao Su

doi:10.1117/12.2534145

17 May 2019 Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit

Meiai Lin, Shuyu Zhang, Qiao Liu, Changshun Shao, Xuantao Su

Author Affiliations +

Proceedings Volume 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019); 1117043 (2019) https://doi.org/10.1117/12.2534145
Event: Fourteenth National Conference on Laser Technology and Optoelectronics, 2019, Shanghai, China

Abstract

The detection of senescent cells becomes increasing important for tumor therapy and drug screening. Here a light sheet microfluidic cytometer with a disposable hydrodynamic focusing unit is developed for two dimensional (2D) light scattering measurements of single cells. The mixed polystyrene microspheres of 3.87 and 2.0 μm in diameter are successfully differentiated by our 2D light scattering microfluidic cytometer. The application of the 2D light scattering microfluidic cytometry for the label-free analysis of senescent cells without any labeling or staining is demonstrated by measurements of H₂O₂-treated U87 cells. Our light sheet-based 2D light scattering microfluidic cytometer is easy to assemble with a disposable hydrodynamic unit, which may find wild applications in clinics for label-free cell classification.

Citation Download Citation

Meiai Lin, Shuyu Zhang, Qiao Liu, Changshun Shao, and Xuantao Su "Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit", Proc. SPIE 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019), 1117043 (17 May 2019); https://doi.org/10.1117/12.2534145

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