Visualization of biological nano-objects with the help of multiphoton microscopy

Olga A. Zakharova; Helena A. Zhambalova; Natalia In. Yunusova; Viktor V. Nikolaev; Alexey V. Borisov

doi:10.1117/12.2540609

18 December 2019 Visualization of biological nano-objects with the help of multiphoton microscopy

Olga A. Zakharova, Helena A. Zhambalova, Natalia In. Yunusova, Viktor V. Nikolaev, Alexey V. Borisov

Proceedings Volume 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics; 112080G (2019) https://doi.org/10.1117/12.2540609
Event: XXV International Symposium, Atmospheric and Ocean Optics, Atmospheric Physics, 2019, Novosibirsk, Russian Federation

Abstract

The results of studies of exosome visualization obtained using multiphoton fluorescence spectroscopy for healthy volunteers and patients with colorectal cancer are presented. Standard centrifuging technology was used to isolate exosomes from blood plasma. Exosome samples were investigated using the femtosecond laser pumping at the wavelength of 760 nm with and without FITC (Fluorescein isothiocyanate) labels. In particular, the exosomes with CD9 surface glycoprotein of the tetraspanin family was shown to be studied without labeling using FLIM (fluorescent lifetime microscopy).

Citation Download Citation

Olga A. Zakharova, Helena A. Zhambalova, Natalia In. Yunusova, Viktor V. Nikolaev, and Alexey V. Borisov "Visualization of biological nano-objects with the help of multiphoton microscopy", Proc. SPIE 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 112080G (18 December 2019); https://doi.org/10.1117/12.2540609

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KEYWORDS

Error control coding

Blood

Fluorescence lifetime imaging

Visualization

Multiphoton microscopy

Luminescence

Proteins

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