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Raster-scan optoacoustic angiography at 532 nm wavelength with 50 µm lateral resolution at 2 mm diagnostic depth was used for quantitative characterization of neoangiogenesis in human colon adenocarcinoma HT-29. Inhomogeneous distribution of areas with high and low vascularization was demonstrated in the tumors. During tumor development vessel growth from the periphery to the center of the tumor was shown. Increase of vascular density preceded the increase of tumor volume.
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Anna Gennadjevna Orlova, Ilya V. Turchin, "Optoacoustic monitoring of angiogenesis in experimental tumors (Conference Presentation)," Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112400P (20 April 2020); https://doi.org/10.1117/12.2550810