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6 March 2020 Photoacoustic and fluorescent microscopy through a multimode fiber by use of optical wavefront shaping (Conference Presentation)
Sylvain Mezil, Irène Wang, Philippe Moreau, Théodore Remark, Antonio M. Caravaca-Aguirre, Emmanuel Bossy
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Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 1124027 (2020) https://doi.org/10.1117/12.2544967
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Many applications of microendoscopy, including brain imaging, requires minimally invasive devices to minimize damage during insertion in the tissue. Here we present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. By learning the transmission matrix during a prior calibration step, a focused spot can be produced and raster-scanned over a sample at the distal tip of the fiber by use of a spatial light modulator. We demonstrate that our setup provides both photoacoustic and fluorescence microscopic images of test samples in vitro (fluorescent beads and red blood cells) through the same fiber.
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Sylvain Mezil, Irène Wang, Philippe Moreau, Théodore Remark, Antonio M. Caravaca-Aguirre, and Emmanuel Bossy "Photoacoustic and fluorescent microscopy through a multimode fiber by use of optical wavefront shaping (Conference Presentation)", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 1124027 (6 March 2020); https://doi.org/10.1117/12.2544967
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KEYWORDS
Multimode fibers
Photoacoustic spectroscopy
Wavefronts
In vitro testing
Microscopy
Tissue optics
Blood
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