Deep-tissue photoacoustic imaging at the second near-infrared region using newly synthesized nickel(II) dithioloene-containing polymeric nanoparticles

Byullee Park; Kyung Min Lee; Misun Yun; Hak-Jong Choi; Jeesu Kim; Changho Lee; Hyungwoo Kim; Chulhong Kim

doi:10.1117/12.2543654

17 February 2020 Deep-tissue photoacoustic imaging at the second near-infrared region using newly synthesized nickel(II) dithioloene-containing polymeric nanoparticles

Byullee Park, Kyung Min Lee, Misun Yun, Hak-Jong Choi, Jeesu Kim, Changho Lee, Hyungwoo Kim, Chulhong Kim

Author Affiliations +

Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112404L (2020) https://doi.org/10.1117/12.2543654
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Extrinsic contrast agents with excellent light absorption properties in the second near-infrared (NIR-II, 1000-1350 nm) region can be a key to enhance the contrast of photoacoustic imaging (PAI) in deep tissues. Here, we demonstrated a photoacoustic (PA) contrast agent at 1064 nm optical wavelength for deep-tissue in vivo PAI. We successfully synthesized nickel(II) dithiolene-based polymeric nanoparticles (NNP) that have strong absorption at NIR-II light and generate improved PA signal with a 1064 nm pulse laser. To confirm the feasibility of the NNP, we have conducted both in vitro and in vivo PA experiments and acquired highly contrast-enhanced PA images. We successfully obtained contrast-enhanced PA images of a tube filled with NNP deeply located below several layers of chicken tissue. The maximum PAI penetration depth was about 5 cm. Next, we performed bladder, sentinel lymph node and gastrointestinal tract, which are clinically important, PAI in rats to confirm that NNP could be utilized as a PA agent in deep tissues in vivo. NNP was injected into each of the three cases, and we confirmed that PA contrast was significantly increased after the injections. These results demonstrate that the enhanced PA signals generated by irradiating 1064 nm laser to NNP in deep-tissue has sufficient contrast for PAI. Based on the excellent absorbability of NNP at 1064 nm and the translability of clinical PAI systems, this study is expected to provide a great opportunity for a variety of studies on non-invasive deep tissue in vivo.

Citation Download Citation

Byullee Park, Kyung Min Lee, Misun Yun, Hak-Jong Choi, Jeesu Kim, Changho Lee, Hyungwoo Kim, and Chulhong Kim "Deep-tissue photoacoustic imaging at the second near-infrared region using newly synthesized nickel(II) dithioloene-containing polymeric nanoparticles", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112404L (17 February 2020); https://doi.org/10.1117/12.2543654

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissues

In vivo imaging

Nanoparticles

Photoacoustic imaging

Polymers

Imaging systems

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years