Development of a time lapse tumor cell spheroid imaging system with a high-resolution spectral-domain optical coherence microscopy system (Conference Presentation)

You-Nan Tsai; Yu-Wei Chang; Chia-Chun Ni; Ting-Hao Chen; Ting-Yen Tsai; Ying-Peng Huang; Chih-Chung Yang; Hsiang-Chieh Lee

doi:10.1117/12.2547049

9 March 2020 Development of a time lapse tumor cell spheroid imaging system with a high-resolution spectral-domain optical coherence microscopy system (Conference Presentation)

You-Nan Tsai, Yu-Wei Chang, Chia-Chun Ni, Ting-Hao Chen, Ting-Yen Tsai, Ying-Peng Huang, Chih-Chung Yang, Hsiang-Chieh Lee

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112431Q (2020) https://doi.org/10.1117/12.2547049
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Living cell culture provides convenient and standard biotechnology chosen option in the laboratory. However, current imaging methods could not present real 3D models. Therefore, we have developed a compact, high-speed spectral-domain optical coherence microscopy (SD-OCM) system to observe the interaction of the tumor cell spheroid with gold nanoparticles. Volumetric OCM images of the cell spheroid were acquired using an in-house C++ interface and used a low-cost microcontroller for triggering to synchronize the galvanometer mirror to the detector array. We designed a hermetic chamber on the microscope stage to control temperature, humidity, CO2 concentration in the experiment.

Conference Presentation

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You-Nan Tsai, Yu-Wei Chang, Chia-Chun Ni, Ting-Hao Chen, Ting-Yen Tsai, Ying-Peng Huang, Chih-Chung Yang, and Hsiang-Chieh Lee "Development of a time lapse tumor cell spheroid imaging system with a high-resolution spectral-domain optical coherence microscopy system (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431Q (9 March 2020); https://doi.org/10.1117/12.2547049

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