A multiplexed confocal FLIM microscope with 4-taps time-gated imager

Morgan Richards; Yuya Shirakawa; Fares Badr; Keiichiro Kagawa; Shoji Kawahito; Qiyin Fang

doi:10.1117/12.2550208

17 February 2020 A multiplexed confocal FLIM microscope with 4-taps time-gated imager

Morgan Richards, Yuya Shirakawa, Fares Badr, Keiichiro Kagawa, Shoji Kawahito, Qiyin Fang

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 1124512 (2020) https://doi.org/10.1117/12.2550208
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a multiplexed confocal FLIM microscope, equipped with a 4-taps time-gated lock-in pixel imager. The multiplexing setup allows the use of the sparse array with sub-nanosecond time-gating to achieve high throughput FLIM acquisition.

Conference Presentation

Citation Download Citation

Morgan Richards, Yuya Shirakawa, Fares Badr, Keiichiro Kagawa, Shoji Kawahito, and Qiyin Fang "A multiplexed confocal FLIM microscope with 4-taps time-gated imager", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124512 (17 February 2020); https://doi.org/10.1117/12.2550208

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