Single molecule localization with four avalanche photodiode detectors

Megan K. Dunlap; Duncan P. Ryan; Peter M. Goodwin; James H. Werner; Somak Majumder; Jennifer A. Hollingsworth; Martin P. Gelfand; Alan Van Orden

doi:10.1117/12.2546573

13 February 2020 Single molecule localization with four avalanche photodiode detectors

Megan K. Dunlap, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Somak Majumder, Jennifer A. Hollingsworth, Martin P. Gelfand, Alan Van Orden

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460O (2020) https://doi.org/10.1117/12.2546573
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Photoluminescence images can be acquired with detection schemes that have both single-photon sensitivity and nanosecond scale temporal resolution, enabling the study of possible structural bases for photoluminescence lifetimes and other features of the photon arrival statistics. Within the context of super-resolution (SR) imaging, this has been demonstrated with detection schemes that collect images with a bundle of optical fibers that are coupled to individual single-photon counting avalanche photodiode detectors. Recently, our group used a bundle of four optical fibers to collect these “time-resolved photon arrival” images. Despite the paucity of information contained in a four-pixel image, we precisely located the emission centroid of quantum dots (QDs) and observed correlations between centroid location, photoluminescence lifetime, and intensity within clusters of QDs that were suggestive of electronic interactions among them. This proceedings paper details the approach that we used to locate the emission centroid based on the counts in the four detectors.

Conference Presentation

Citation Download Citation

Megan K. Dunlap, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Somak Majumder, Jennifer A. Hollingsworth, Martin P. Gelfand, and Alan Van Orden "Single molecule localization with four avalanche photodiode detectors", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460O (13 February 2020); https://doi.org/10.1117/12.2546573

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available