Stimulated Raman scattering - vibrational imaging in cells, tissues and model organisms (Conference Presentation)

Volker Schweikhard

doi:10.1117/12.2544013

10 March 2020 Stimulated Raman scattering - vibrational imaging in cells, tissues and model organisms (Conference Presentation)

Volker Schweikhard

Author Affiliations +

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112521J (2020) https://doi.org/10.1117/12.2544013
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Stimulated Raman Scattering (SRS) microscopy is a powerful nonlinear optical microscopy technique that images biological structures by exploiting the characteristic, vibrational contrast of the sample molecules. SRS provides a rich, chemically specific and biophysical image contrast that is in many ways complementary to the molecular contrast of fluorescence microscopy. Here, we present a range of applications of SRS, including label-free morphochemical imaging in model organisms, the characterization of organoids and spheroids, and investigations of brain tissues for neurodegenerative disease research. We show specifically that SRS can provide novel insights into the biophysical properties and biochemical composition of Amyloid-β plaques in a mouse model of Alzheimer’s disease. Our results highlight the potential of SRS to contribute to a deeper understanding of cell and tissue biology, and to serve as a powerful tool for preclinical and translational research.

Conference Presentation

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Volker Schweikhard "Stimulated Raman scattering - vibrational imaging in cells, tissues and model organisms (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521J (10 March 2020); https://doi.org/10.1117/12.2544013

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KEYWORDS

Tissues

Organisms

Raman scattering

Microscopy

Brain

Luminescence

Molecules

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