Hyper-raman spectroscopy of hydrogen bonding in DMSO-water mixtures

Xingqi Xu; Christopher B. Marble; Georgi I. Petrov; Dawei Wang; Vladislav V. Yakovlev

doi:10.1117/12.2545496

26 February 2020 Hyper-raman spectroscopy of hydrogen bonding in DMSO-water mixtures

Xingqi Xu, Christopher B. Marble, Georgi I. Petrov, Dawei Wang, Vladislav V. Yakovlev

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112521T (2020) https://doi.org/10.1117/12.2545496
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Dimethyl sulfoxide (DMSO) is a biologically important solvent in part due to its dual miscibility with hydrophilic and hydrophobic molecules. Binary solutions of DMSO-water display non-ideal thermodynamics properties such as high viscosity and low freezing point due to hydrogen bonding. The unusual properties of DMSO-water solutions have been exploited to disrupt the formation of secondary structures of proteins during polymerase chain reaction assays and to act as a cryoprotectant for tissues. The exact coordination of the DMSO and water molecules remains unknown. Hyper- Raman scattering was employed for the first time to investigate binary systems of DMSO with water (H₂O). As a part of this study, hyper-Raman and Raman spectra of pure solutions were first acquired and compared against existing Raman and IR spectroscopic data. Then the corresponding measurements were taken with deuterated DMSO-d₆ and heavy water (D₂O) to validate the analysis and to isolate overlapping spectral features. The permissive selection rules of hyper- Raman scattering provide new insight into disruptions of the self-hydrogen bonded networks of DMSO and water and the establishment of hydrogen bonded networks.

Citation Download Citation

Xingqi Xu, Christopher B. Marble, Georgi I. Petrov, Dawei Wang, and Vladislav V. Yakovlev "Hyper-raman spectroscopy of hydrogen bonding in DMSO-water mixtures", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521T (26 February 2020); https://doi.org/10.1117/12.2545496

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KEYWORDS

Hydrogen

Molecules

Spectroscopy

Raman spectroscopy

Infrared spectroscopy

Liquids

Raman scattering

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