Tracing the formation and degradation of fatty-acid-rich mitochondria using label-free chemical imaging (Conference Presentation)

Chi Zhang; Stephen A. Boppart

doi:10.1117/12.2546083

10 March 2020 Tracing the formation and degradation of fatty-acid-rich mitochondria using label-free chemical imaging (Conference Presentation)

Chi Zhang, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112521V (2020) https://doi.org/10.1117/12.2546083
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Using coherent anti-Stokes Raman scattering (CARS) microscopy, we discovered heterogeneity of mitochondrial chemical compositions in cancer cells after hypothermia. Individual mitochondria associated with higher signals in CARS images are likely the result of fatty acid accumulation, which is caused by a reduced rate of fatty acid β-oxidation. Tracing individual mitochondria after reheating the cells to 37°C reveals degradation of these organelles through the mitophagy process. Further study will continue to unveil how stressed mitochondria would form in various conditions, and the fate of these organelles upon changes of their environment. Our results shed new light on mitochondrial function and cell metabolism.

Conference Presentation

Citation Download Citation

Chi Zhang and Stephen A. Boppart "Tracing the formation and degradation of fatty-acid-rich mitochondria using label-free chemical imaging (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521V (10 March 2020); https://doi.org/10.1117/12.2546083

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