A practical theoretical framework for optimizing spontaneous super-resolution on confocal microscopes (Conference Presentation)

Martin Ploschner; Denitza Denkova; Minakshi Das; Lindsay M. Parker; Xianlin Zheng; Yiqing Lu; Antony Orth; Nicolle H. Packer; James A. Piper

doi:10.1117/12.2544972

10 March 2020 A practical theoretical framework for optimizing spontaneous super-resolution on confocal microscopes (Conference Presentation)

Martin Ploschner, Denitza Denkova, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Antony Orth, Nicolle H. Packer, James A. Piper

Author Affiliations +

Proceedings Volume 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII; 112540E (2020) https://doi.org/10.1117/12.2544972
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Recently, we realized a simple technique, which spontaneously bypasses the diffraction limit in a conventional confocal microscope by exploiting super-linear effects in nanoparticle bio-markers: super-linear excitation-emission (SEE) microscopy. Here, we present a theoretical framework and its practical implementation for optimizing and expanding this technique. We accurately predict the expected 3D super-resolution by accounting for all crucial parameters affecting the resolution: the empirically measured/modelled excitation-emission curve, the filling factor of the microscope objective back pupil, the polarization and the pinhole setting. The presented theoretical framework is a practical tool, which enables end-users to augment their own confocal microscopes with super-resolution capabilities.

Conference Presentation

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Martin Ploschner, Denitza Denkova, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Antony Orth, Nicolle H. Packer, and James A. Piper "A practical theoretical framework for optimizing spontaneous super-resolution on confocal microscopes (Conference Presentation)", Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540E (10 March 2020); https://doi.org/10.1117/12.2544972

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KEYWORDS

Microscopes

Microscopy

Confocal microscopy

Nanoparticles

Diffraction

Optical microscopes

Bacteria

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