Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.

B. Adinolfi; S. Tombelli; C. Trono; A. Giannetti; M. Pellegrino; G. Sotgiu; G. Varchi; M. Ballestri; F. Baldini

doi:10.1117/12.2547604

21 February 2020 Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.

B. Adinolfi, S. Tombelli, C. Trono, A. Giannetti, M. Pellegrino, G. Sotgiu, G. Varchi, M. Ballestri, F. Baldini

Author Affiliations +

Proceedings Volume 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII; 112540H (2020) https://doi.org/10.1117/12.2547604
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

One of the main goals in nanomedicine is the development of effective drug delivery systems. Polymeric nanoparticles (NPs) have been exploited as nanocarriers for their stability, handiness and biocompatibility. In this context, NPs have been coupled to molecular beacons (MBs), theranostic agents that conjugate the sensing of specific mRNA with a silencing activity. The aim of this work was to evaluate by confocal microscopy and fluorescence measurements: a) the involvement of endocytosis in the NP uptake; b) the NP fate at different times of cell incubation to verify their localization in lysosomes; c) the MB localization on the Endoplasmic Reticulum (ER) where the target mRNA is located. Results: a) PMMA-NPs promote endocytosis; b) strong co-localization of NP and lysosome tracker fluorescence after 2- 48 hours of incubation; c) co-localization of the MB fluorescence with the ER-marker signal after 90' of incubation. The data highlight the ability of the PMMA-NPs to promote the survivin-MB internalization by endocytosis and demonstrate that the PMMA-NPs are an appropriate delivery system capable of being eliminated by cells; these evidences also contribute to consider the MB as an effective tool for the intracellular sensing.

Conference Presentation

Citation Download Citation

B. Adinolfi, S. Tombelli, C. Trono, A. Giannetti, M. Pellegrino, G. Sotgiu, G. Varchi, M. Ballestri, and F. Baldini "Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.", Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540H (21 February 2020); https://doi.org/10.1117/12.2547604

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available