As a non-invasive imaging tool, second harmonic generation (SHG) imaging has shown a great prospect in the visualization of living cells and tissues. It is widely used in the fields of science, medicine, biology and tissue engineering. Although the SHG intensity is often used as the basis of imaging analysis, its interpretation is still very subjective. In this project, the nonlinear process behind SHG is described. In order to explore the wavelength-dependence of SHG, the simulation experiments are carried out. A contrast model is used to calculate the forward- and backward- generated SHG signals. The simulation results show the SHG intensities of different collagen models at the wavelength between 760 nm to 1000 nm.
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