Improvement of imaging quality of Fourier ptychographic microscopy

Yan Sun; Dayong Wang; Yunxin Wang; Jie Zhao; Lu Rong

doi:10.1117/12.2549199

12 March 2020 Improvement of imaging quality of Fourier ptychographic microscopy

Yan Sun, Dayong Wang, Yunxin Wang, Jie Zhao, Lu Rong

Proceedings Volume 11434, 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments; 1143412 (2020) https://doi.org/10.1117/12.2549199
Event: 2019 International Conference on Optical Instruments and Technology, 2019, Beijing, China

Abstract

In the present of microscopic imaging technology, both the large imaging field of view (FOV), and high-resolution are required. The Fourier ptychographic microscopy (FPM) does not need to move the sample and can keep the original FOV unchanged. It can expand the frequency bandwidth and reconstruct high-resolution intensity and phase images by using the angle diversity and synthetic aperture. It has a very important application prospect in the field of biomedical detection. However, the LED, used as the illumination source in the FPM, is an extended light source, and the light emitting unit with a certain size will cause the image blurring. Therefore, a reconstruction algorithm based on Lucy-Richardson (L-R) deconvolution is proposed in this paper. The experimental results show that the proposed algorithm can solve the problem of image blurring in FPM imaging and improve the imaging quality.

Citation Download Citation

Yan Sun, Dayong Wang, Yunxin Wang, Jie Zhao, and Lu Rong "Improvement of imaging quality of Fourier ptychographic microscopy", Proc. SPIE 11434, 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 1143412 (12 March 2020); https://doi.org/10.1117/12.2549199

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KEYWORDS

Reconstruction algorithms

Deconvolution

Light emitting diodes

Microscopy

Image processing

Image quality

Imaging systems

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