A new method for separate reconstruction of fluorophore absorption and fluorescence lifetime in time domain: first results of a numerical experiment

Alexander B. Konovalov; Vitaly V. Vlasov

doi:10.1117/12.2560139

9 April 2020 A new method for separate reconstruction of fluorophore absorption and fluorescence lifetime in time domain: first results of a numerical experiment

Alexander B. Konovalov, Vitaly V. Vlasov

Author Affiliations +

Proceedings Volume 11457, Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine; 1145703 (2020) https://doi.org/10.1117/12.2560139
Event: Saratov Fall Meeting 2019: VII International Symposium on Optics and Biophotonics, 2019, Saratov, Russian Federation

Abstract

The paper is devoted to a new method of time-domain lifetime fluorescence molecular tomography. It is based on the use of early arriving diffuse photons and original measurement data, which are determined for the individual time gates of the diffuse temporal responses for fluorescence and exiting radiation. The paper presents in short the theoretical foundation of the method and describes a numerical experiment on the separate reconstruction of fluorophore absorption coefficient and fluorescence lifetime for 3D reflectance geometry that is suitable for small animal imaging. We show our first reconstruction results for the 3D scattering object region 11×11×8 mm³ in size with two spherical fluorescent inclusions 1 mm in diameter. The ways how to improve these results in the future are discussed.

Citation Download Citation

Alexander B. Konovalov and Vitaly V. Vlasov "A new method for separate reconstruction of fluorophore absorption and fluorescence lifetime in time domain: first results of a numerical experiment", Proc. SPIE 11457, Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine, 1145703 (9 April 2020); https://doi.org/10.1117/12.2560139

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