Metamaterial assisted cellular imaging using photobleaching kinetics

Yeon Ui Lee; Junxiang Zhao; Gary C. H. Mo; Shilong Li; Guangru Li; Qian Ma; Qingqing Yang; Ratnesh Lal; Jin Zhang; Zhaowei Liu

doi:10.1117/12.2568896

20 August 2020 Metamaterial assisted cellular imaging using photobleaching kinetics

Yeon Ui Lee, Junxiang Zhao, Gary C. H. Mo, Shilong Li, Guangru Li, Qian Ma, Qingqing Yang, Ratnesh Lal, Jin Zhang, Zhaowei Liu

Author Affiliations +

Proceedings Volume 11468, Enhanced Spectroscopies and Nanoimaging 2020; 114680F (2020) https://doi.org/10.1117/12.2568896
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

In the past two decades, various super-resolution fluorescence microscopic techniques have achieved an axial resolution on the order of tens of nanometers and been applied for a wide range of biological studies. However, these imaging techniques still face technical challenges to reach a resolution below 10 nm. Moreover, the required complex system for these techniques limits their wide applications in practice. In this talk, we present a new cellular fluorescence imaging method with a nanometer-scale axial resolution, based on a distance-dependent photobleaching suppression of fluorophores on hyperbolic metamaterial. We will show that by applying this technology to image HeLa cell membranes tagged with fluorescent proteins, an axial resolution of ~3 nm at multiple colors can be achieved, allowing for a precise determination of the architecture of cell adhesion.

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Citation Download Citation

Yeon Ui Lee, Junxiang Zhao, Gary C. H. Mo, Shilong Li, Guangru Li, Qian Ma, Qingqing Yang, Ratnesh Lal, Jin Zhang, and Zhaowei Liu "Metamaterial assisted cellular imaging using photobleaching kinetics", Proc. SPIE 11468, Enhanced Spectroscopies and Nanoimaging 2020, 114680F (20 August 2020); https://doi.org/10.1117/12.2568896

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