Towards single-photon deep-tissue microscopy

Tom Vettenburg

doi:10.1117/12.2573210

15 June 2020 Towards single-photon deep-tissue microscopy

Tom Vettenburg

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152103 (2020) https://doi.org/10.1117/12.2573210
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope’s ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.

Citation Download Citation

Tom Vettenburg "Towards single-photon deep-tissue microscopy", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152103 (15 June 2020); https://doi.org/10.1117/12.2573210

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KEYWORDS

Microscopy

Luminescence

Tissues

Light scattering

Image enhancement

Microscopes

Adaptive optics

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