A surface-enhanced Raman scattering molecular beacon for the detection of miRNA-21

Zhile Wang; Kai Zhu; Shenfei Zong; Zhuyuan Wang; Yiping Cui

doi:10.1117/12.2585146

10 October 2020 A surface-enhanced Raman scattering molecular beacon for the detection of miRNA-21

Zhile Wang, Kai Zhu, Shenfei Zong, Zhuyuan Wang, Yiping Cui

Proceedings Volume 11554, Advanced Sensor Systems and Applications X; 115541Q (2020) https://doi.org/10.1117/12.2585146
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

A surface-enhanced Raman scattering (SERS) based microRNA (miRNA) probe was designed using molecular beacon (MB). In such a probe, silver nanoparticles were employed as the SERS substrate while 6-FAM was selected as a Raman reporter, which were connected by a hairpin shaped DNA. In the presence of target miRNA, the hairpin opened, making 6-FAM far away from the substrate with a decreased SERS intensity. As the concentration of the targets increased, the SERS intensity of Raman reporters decreased. Thus, according to the intensity-concentration calibration curve, the detection of miRNA-21 could be realized. The convenient, stable and sensitive detection method holds potential in the application of diagnosis.

Conference Presentation

Citation Download Citation

Zhile Wang, Kai Zhu, Shenfei Zong, Zhuyuan Wang, and Yiping Cui "A surface-enhanced Raman scattering molecular beacon for the detection of miRNA-21", Proc. SPIE 11554, Advanced Sensor Systems and Applications X, 115541Q (10 October 2020); https://doi.org/10.1117/12.2585146

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