Optical fluence-compensated functional optical-resolution photoacoustic microscopy

Jingyi Zhu; Chao Liu; Jiangbo Chen; Yachao Zhang; Lidai Wang

doi:10.1117/12.2577491

5 March 2021 Optical fluence-compensated functional optical-resolution photoacoustic microscopy

Jingyi Zhu, Chao Liu, Jiangbo Chen, Yachao Zhang, Lidai Wang

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116421P (2021) https://doi.org/10.1117/12.2577491
Event: SPIE BiOS, 2021, Online Only

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) can image the blood oxygen saturation (sO₂) in vivo without labeling. OR-PAM assumes a linear relationship between the photoacoustic amplitude and the optical absorption coefficient and ignores the wavelength-dependent optical fluence attenuation in tissue. However, strong scattering in biological tissues may significantly change the optical energy deposition, leading to inaccurate sO₂ measurement. Here, we report fluence-compensated OR-PAM to correct the sO₂ imaging. In a narrow optical spectrum, we assume the scattered fluence is linearly related to the optical wavelength. Using three optical wavelengths, we can compensate for the scattering-induced photoacoustic signal change and thus improve the accuracy of sO₂ measurement. We use a Monta Carlo model to validate the linear assumption of the scattered fluence. In in vivo experiments, we demonstrate that the optical fluence compensation can effectively improve the sO₂ accuracy. The compensated arterial sO₂ values are in the range of 0.95 ~ 0.99, which is consistent with normal physiological values. Compared with the uncompensated ones, the accuracy has been improved greatly. Enabled by the accurate sO₂ imaging tool, we can reliably observe the sO₂ gradient in the vascular network. We expect this new technique will further broaden the preclinical and clinical applications of functional OR-PAM.

Conference Presentation

Citation Download Citation

Jingyi Zhu, Chao Liu, Jiangbo Chen, Yachao Zhang, and Lidai Wang "Optical fluence-compensated functional optical-resolution photoacoustic microscopy", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116421P (5 March 2021); https://doi.org/10.1117/12.2577491

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