Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen; Yachao Zhang; Linyun He; Yizhi Liang; Lidai Wang

doi:10.1117/12.2577486

5 March 2021 Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen, Yachao Zhang, Linyun He, Yizhi Liang, Lidai Wang

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422U (2021) https://doi.org/10.1117/12.2577486
Event: SPIE BiOS, 2021, Online Only

Abstract

We report wide-field polygon-scanning optical-resolution photoacoustic microscopy (OR-PAM) that achieves 1-MHz A-line rate of oxygen saturation in vivo. We develop a polygon-scanning imaging probe. Using stimulated Raman scattering and optical delay in fiber, we develop a dual-wavelength pulsed laser that has proper wavelengths, sufficient pulse energy, high pulse repetition rate, and sub-microseconds wavelength switching time for fast imaging of oxygen saturation. The OR-PAM system offers a 478-Hz B-scan rate and a 1-Hz volumetric imaging rate over a 12×5 mm² scanning area. We demonstrate dynamic imaging of oxygen saturation of fast response to epinephrine injection in the mouse ear. Results show fast hemodynamic and functional changes in centimeter-sized areas.

Conference Presentation

Citation Download Citation

Jiangbo Chen, Yachao Zhang, Linyun He, Yizhi Liang, and Lidai Wang "Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422U (5 March 2021); https://doi.org/10.1117/12.2577486

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