Quantification of light localization properties to study the effect of probiotic on chronic alcoholic brain cells via confocal imaging

Prakash Adhikari; Pradeep K. Shukla; Radhakrishna Rao; Prabhakar Pradhan

doi:10.1117/12.2578785

5 March 2021 Quantification of light localization properties to study the effect of probiotic on chronic alcoholic brain cells via confocal imaging

Prakash Adhikari, Pradeep K. Shukla, Radhakrishna Rao, Prabhakar Pradhan

Author Affiliations +

Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 1164716 (2021) https://doi.org/10.1117/12.2578785
Event: SPIE BiOS, 2021, Online Only

Abstract

Photonics localization due to light scattering is an important probe to understand the molecular specific structural changes in brain cells due to diseases or abnormalities. Chronic alcoholism is associated with medical, behavioral, and psychological problems including physical damage in brain cells/tissues. The effects of chronic alcoholism on brain cells/tissue structures at the nanoscale are not well understood. On the other hand, probiotic treatment has shown some reversing effect in curing the abnormalities in an alcoholic’s brain. In this work, we study the effect of probiotic, Lactobacillus Plantarum treatment on chronic alcoholic brain cells/tissues using a mouse model. We evaluate the light localization properties of molecular specific spatial mass density fluctuations based on mesoscopic physics-based inverse participation ratio via confocal imaging of cells, confocal-IPR technique. Using the technique, we probe overexpression of astrocyte and microglial cells, and chromatin structures of different brain cells, by probing molecular specific overexpression by staining the cells with appropriate dye/proteins and then calculating the degree of spatial molecular structural disorder (Ld). The confocal-IPR analysis of alcoholic astrocytes, microglia, and chromatin of the mice brain cells show an increase in the structural disorder indicating that alcohol has an adverse effect on different brain cells and nuclei. Whereas the normalcy in the structural disorder of these brain cells happens when probiotics were fed simultaneously with alcohol, confirms the improvement in chronic alcoholic brain health. The potential application of this novel approach to diagnosing the alcohol effect and probiotic treatment in the alcoholic brain is explored.

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Prakash Adhikari, Pradeep K. Shukla, Radhakrishna Rao, and Prabhakar Pradhan "Quantification of light localization properties to study the effect of probiotic on chronic alcoholic brain cells via confocal imaging", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 1164716 (5 March 2021); https://doi.org/10.1117/12.2578785

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