Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux

Ikbal Sencan; Wenjing Zong; Mohammed A. Alfadhel; Buyin Fu; Mohammad A. Yaseen; Gary D. Wu; Sava Sakadzic; Sergei A. Vinogradov

doi:10.1117/12.2583123

5 March 2021 Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux

Ikbal Sencan, Wenjing Zong, Mohammed A. Alfadhel, Buyin Fu, Mohammad A. Yaseen, Gary D. Wu, Sava Sakadzic, Sergei A. Vinogradov

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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480H (2021) https://doi.org/10.1117/12.2583123
Event: SPIE BiOS, 2021, Online Only

Abstract

Quantifying intestinal microvascular oxygenation may shed light on origin and progression of intestinal diseases as well as the role of the gut microbiota. In this work, we developed a method for depth-resolved microscopic measurements of partial pressure of oxygen (pO2) and Red Blood Cell (RBC) flux in the capillaries of intestinal wall in mouse cecum. Capillary pO2 and RBC flux were measured in healthy mice as a function of depth during normoxia and hyperoxia. Our results show low mean capillary RBC flux and relatively uniform capillary pO2 across the cecum wall depth.

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Ikbal Sencan, Wenjing Zong, Mohammed A. Alfadhel, Buyin Fu, Mohammad A. Yaseen, Gary D. Wu, Sava Sakadzic, and Sergei A. Vinogradov "Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480H (5 March 2021); https://doi.org/10.1117/12.2583123

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KEYWORDS

Capillaries

Microscopy

Phosphorescence

Oxygen

Body temperature

Hyperoxia

Intestine

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