Programmable LED array reflectance microscopy for multi-contrast imaging

Weiye Song; Alex Matlock; Sipei Fu; Xiaodan Qin; Hui Feng; Christopher V. Gabel; Lei Tian; Ji Yi

doi:10.1117/12.2578905

5 March 2021 Programmable LED array reflectance microscopy for multi-contrast imaging

Weiye Song, Alex Matlock, Sipei Fu, Xiaodan Qin, Hui Feng, Christopher V. Gabel, Lei Tian, Ji Yi

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116530H (2021) https://doi.org/10.1117/12.2578905
Event: SPIE BiOS, 2021, Online Only

Abstract

LED array microscopy is an emerging platform for computational imaging . Existing LED array systems often exploit transmission imaging geometries of standard brightfield microscopes that leave the rich backscattered field undetected. Here, we develop an LED array reflectance microscope capturing the sample’s backscattered signal. In particular, we demonstrate multimodal brightfield, darkfield, and differential phase contrast imaging on fixed and living biological specimens including Caenorhabditis elegans (C. elegans), zebrafish embryos, and live cell cultures. Video-rate multimodal imaging records real-time features of freely-moving C. elegans and the fast beating heart of zebrafish embryo. Our new reflectance mode is a valuable addition to the LED array microscopic toolbox.

Conference Presentation

Citation Download Citation

Weiye Song, Alex Matlock, Sipei Fu, Xiaodan Qin, Hui Feng, Christopher V. Gabel, Lei Tian, and Ji Yi "Programmable LED array reflectance microscopy for multi-contrast imaging", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116530H (5 March 2021); https://doi.org/10.1117/12.2578905

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