Label-free three-dimensional imaging and quantitative analysis of long-term on-chip vasculogenesis using optical diffraction tomography

Chungha Lee; Seunggyu Kim; Herve Hugonnet; Jessie S. Jeon; WeiSun Park; YongKeun Park; Moosung Lee

doi:10.1117/12.2584889

5 March 2021 Label-free three-dimensional imaging and quantitative analysis of long-term on-chip vasculogenesis using optical diffraction tomography

Chungha Lee, Seunggyu Kim, Herve Hugonnet, Jessie S. Jeon, WeiSun Park, YongKeun Park, Moosung Lee

Author Affiliations +

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116531S (2021) https://doi.org/10.1117/12.2584889
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Three-dimensional (3D), label-free, and long-term imaging in organ-on-a-chip research is necessary to assess the dynamic development of multicellular structures inside a microfluidic chip and relevant physiological functions. However, imaging on-chip multicellular landscapes generally relies on the use of exogenous labels, which limits the long-term imaging of live cells due to photobleaching and phototoxicity. Here, we demonstrate the 3D label-free observation of 5-day on-chip vasculogenesis of human umbilical vein endothelial cells using optical diffraction tomography. The proposed method enables us to quantify both morphological and biochemical alterations during the vasculogenesis.

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Chungha Lee, Seunggyu Kim, Herve Hugonnet, Jessie S. Jeon, WeiSun Park, YongKeun Park, and Moosung Lee "Label-free three-dimensional imaging and quantitative analysis of long-term on-chip vasculogenesis using optical diffraction tomography", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116531S (5 March 2021); https://doi.org/10.1117/12.2584889

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