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5 March 2021 Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume
Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, Rainer Erdmann
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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560H (2021) https://doi.org/10.1117/12.2576400
Event: SPIE BiOS, 2021, Online Only
Abstract
We will demonstrate the performance of a spectrometer-microscope assembly for characterization and analysis of samples in terms of lifetime, spectral and spatial resolution. This combined approach provides access to further information, which are not available when using only lifetime or steady-state experiments. The combination of both techniques in one setup can help to understand biochemical or physical processes by detecting changes in local environment such as pH, temperature, or ion concentration, and to identify molecular interactions or conformation changes via Förster Resonance Energy Transfer (FRET).
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Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, and Rainer Erdmann "Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560H (5 March 2021); https://doi.org/10.1117/12.2576400
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KEYWORDS
Luminescence
Spectroscopy
Molecular spectroscopy
Molecules
Statistical analysis
Proteins
Resonance energy transfer
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