Sub-second low frequency vibrational SRS imaging using a fast acousto-optic delay line

Siddarth Shivkumar; Dekel Raanan; Xavier Audier; Maor Asher; Matan Menahem; Omer Yaffe; Nicolas Forget; Dan Oron; Hervé Rigneault

doi:10.1117/12.2576610

5 March 2021 Sub-second low frequency vibrational SRS imaging using a fast acousto-optic delay line

Siddarth Shivkumar, Dekel Raanan, Xavier Audier, Maor Asher, Matan Menahem, Omer Yaffe, Nicolas Forget, Dan Oron, Hervé Rigneault

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560X (2021) https://doi.org/10.1117/12.2576610
Event: SPIE BiOS, 2021, Online Only

Abstract

Low frequency vibrations (< 200cm^(-1)) contain the structural information of molecules or the crystal lattice - making low frequency Raman imaging an ideal candidate to analyze heavy molecules, crystal formation etc. The longer integration times of spontaneous Raman spectroscopy, however, limits the study of dynamic structural changes. In our work, we have built a stimulated Raman scattering (SRS) pump probe scheme combining impulsive excitation with a fast acousto-optics delay line. With a pixel dwell time of 25μs, we have acquired, on a shot noise limited detection system, sub second low frequency (< 200cm^(-1)) hyper-spectral SRS images of various samples.

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Siddarth Shivkumar, Dekel Raanan, Xavier Audier, Maor Asher, Matan Menahem, Omer Yaffe, Nicolas Forget, Dan Oron, and Hervé Rigneault "Sub-second low frequency vibrational SRS imaging using a fast acousto-optic delay line", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560X (5 March 2021); https://doi.org/10.1117/12.2576610

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