Optical monitoring of L-DNA enantiomers in PCR: application to "unextracted" COVID-19 specimens

Christia M. Victoriano; Mindy Leelawong; Frederick R. Haselton; Nicholas Adams

doi:10.1117/12.2577282

5 March 2021 Optical monitoring of L-DNA enantiomers in PCR: application to "unextracted" COVID-19 specimens

Christia M. Victoriano, Mindy Leelawong, Frederick R. Haselton, Nicholas Adams

Proceedings Volume 11662, Frontiers in Biological Detection: From Nanosensors to Systems XIII; 1166209 (2021) https://doi.org/10.1117/12.2577282
Event: SPIE BiOS, 2021, Online Only

Abstract

The ongoing COVID-19 pandemic highlights the need for simple and rapid testing for respiratory infections at the point-of-care. With its high sensitivity and specificity, RT-PCR is the gold standard for the molecular diagnosis and differentiation among respiratory pathogens, but it requires complex instrumentation and methods that are not suitable for point-of-care settings. Our simplified Adaptive PCR technology and workflow yields high analytic performance without the complexities of traditional PCR by incorporating mirror-image L-DNA enantiomers—identical in sequence to PCR primers and targets—that are optically monitored to adapt cycling conditions to match the biochemical contents of the sample in real time. This enables rapid, single-tube analyses directly from COVID-19 swab specimens without RNA extraction.

Conference Presentation

Citation Download Citation

Christia M. Victoriano, Mindy Leelawong, Frederick R. Haselton, and Nicholas Adams "Optical monitoring of L-DNA enantiomers in PCR: application to "unextracted" COVID-19 specimens", Proc. SPIE 11662, Frontiers in Biological Detection: From Nanosensors to Systems XIII, 1166209 (5 March 2021); https://doi.org/10.1117/12.2577282

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