Effects of substrate stiffness on the mechanical characteristics of breast cells using atomic force microscopy

Yan Zhang; Xiaoqiong Tang; Aisi Shi; Yuhua Wang; Zhengchao Wang; Hongqin Yang

doi:10.1117/12.2600613

15 October 2021 Effects of substrate stiffness on the mechanical characteristics of breast cells using atomic force microscopy

Yan Zhang, Xiaoqiong Tang, Aisi Shi, Yuhua Wang, Zhengchao Wang, Hongqin Yang

Author Affiliations +

Proceedings Volume 11896, Advanced Optical Imaging Technologies IV; 118960O (2021) https://doi.org/10.1117/12.2600613
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Conference Poster

Abstract

The development of tumor is closely related to extracellular matrix, which changes the biomechanical behavior of cells.Research have prepared polyacrylamide hydrogel substrates of differing stiffness according to the hardness values of breast tissue under normal and tumor physiological conditions. Then AFM was used to measure the mechanical properties of breast cells with different degrees of malignancy grown on different stiffness substrates. To explore the reasons for the changes in the young’s modulus of three breast cells, the distribution of cellular actin filaments were observed with a confocal microscope. These results showed that when the substrate hardened, the viscoelasticity of benign breast cells increased significantly, and the other two cancer cells also changed to some extent. We also found that the harder the substrate, the more conducive to the spreading behavior of cells, and the weaker response of malignant cells to substrates.

Conference Presentation

Citation Download Citation

Yan Zhang, Xiaoqiong Tang, Aisi Shi, Yuhua Wang, Zhengchao Wang, and Hongqin Yang "Effects of substrate stiffness on the mechanical characteristics of breast cells using atomic force microscopy", Proc. SPIE 11896, Advanced Optical Imaging Technologies IV, 118960O (15 October 2021); https://doi.org/10.1117/12.2600613

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available