Multimodal optical monitoring of auto and allografts of skin on a burn wound

Maksim Ryabkov; Vladimir Beschastnov; Ksenia Petrova; Petr Peretyagin; Anna Orlova; Aleksey Kostyuk; Ilya Turchin; Marina Bugrova; Igor Yu Arefiev

doi:10.1117/12.2609580

3 March 2022 Multimodal optical monitoring of auto and allografts of skin on a burn wound

Maksim Ryabkov, Vladimir Beschastnov, Ksenia Petrova, Petr Peretyagin, Anna Orlova, Aleksey Kostyuk, Ilya Turchin, Marina Bugrova, Igor Yu Arefiev

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Proceedings Volume 11934, Photonics in Dermatology and Plastic Surgery 2022; 1193402 (2022) https://doi.org/10.1117/12.2609580
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Objective: to study the dynamics of the state of autografts of skin and allodermal protectors on a wound using multimodal optical monitoring. Material and methods. A burn wound was simulated in rats (n = 16), 20% of the wound area was covered with skin autografts. The allodermal protector of 0.35 mm thick was applied over the autografts. Studied in vivo the state of the grafts for 10 days: saturation - according to diffuse optical spectroscopy (DOS); perfusion - according to laser Doppler flowmetry (LDF); microstructure - according to optical coherence tomography (OCT). Results. Multimodal monitoring of blood circulation, metabolism and microstructure of skin grafts on a burn wound showed that changes in auto- and allografts occur asynchronously. In the tissues of the autograft, blood saturation directly correlated with the restoration of perfusion (Spearman's coefficient = 0.795); in the allograft, the correlation between perfusion and saturation was weakly inverse (-0.179). Those differences were confirmed by OCT data and histological analysis: allografts lost their normal microstructure simultaneously with a rapid decrease of the blood saturation, despite the preservation of perfusion parameters.

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Maksim Ryabkov, Vladimir Beschastnov, Ksenia Petrova, Petr Peretyagin, Anna Orlova, Aleksey Kostyuk, Ilya Turchin, Marina Bugrova, and Igor Yu Arefiev "Multimodal optical monitoring of auto and allografts of skin on a burn wound", Proc. SPIE 11934, Photonics in Dermatology and Plastic Surgery 2022, 1193402 (3 March 2022); https://doi.org/10.1117/12.2609580

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