Photoswitchable fluorescence is a powerful technique to realize super-resolution imaging, highlighting, and optical storage, while its multiplexing capability is limited. Raman scattering is attracting attention because it generates narrowband vibrational signatures, which are potentially useful for highly multiplexed detection of different constituents. Here, we demonstrate photoswitchable SRS spectroscopy and microscopy assisted by photochromic molecules. The narrowband Raman signatures can be switched with full reversibility by applying the irradiation of UV or visible light. The switching speed under low power irradiation is evaluated as fast as within 1 microsecond which is compatible with a conventional point-scan microscope. The demonstration of live-cell imaging suggests the good compatibility to living systems and satisfying sensitivity of this method. We anticipate that photoswitchable SRS imaging will be a powerful foundation for super-multiplex super-resolution imaging.
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