Multimodal multiphoton microscope with depth scanning

Wentao Wu; Qihao Liu; Christoph Brandt; Shuo Tang

doi:10.1117/12.2608365

3 March 2022 Multimodal multiphoton microscope with depth scanning

Wentao Wu, Qihao Liu, Christoph Brandt, Shuo Tang

Proceedings Volume 11965, Multiphoton Microscopy in the Biomedical Sciences XXII; 119650J (2022) https://doi.org/10.1117/12.2608365
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. An Er-doped fiber laser with 1580 nm and 790 nm output provides the dual-wavelength multimodal capability and a shape-memory-alloy (SMA) based depth-scanning objective enables the depth scanning. Image stacks combining two-photon-excitation-fluorescence (TPEF), second-harmonic-generation (SHG), and third-harmonic-generation (THG) signals have been acquired on animal samples from the surface to over 200 μm underneath.

Citation Download Citation

Wentao Wu, Qihao Liu, Christoph Brandt, and Shuo Tang "Multimodal multiphoton microscope with depth scanning", Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, 119650J (3 March 2022); https://doi.org/10.1117/12.2608365

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