Fast, cell-resolution, wide field-of-view two-photon microscopy to reveal functional network architectures across multi-modal cortical areas

Masanori Murayama

doi:10.1117/12.2612749

2 March 2022 Fast, cell-resolution, wide field-of-view two-photon microscopy to reveal functional network architectures across multi-modal cortical areas

Masanori Murayama

Author Affiliations +

Proceedings Volume 11971, High-Speed Biomedical Imaging and Spectroscopy VII; 1197103 (2022) https://doi.org/10.1117/12.2612749
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Fast brain-wide imaging with single-cell resolution, high signal-to-noise ratio, and no optical aberrations have the potential to inspire new avenues of investigations in biology. However, such imaging is challenging because of the inevitable tradeoffs among these parameters. Here, we overcome these tradeoffs by combining a resonant scanning system, a large objective with low magnification and high numerical aperture, and highly sensitive large-aperture photodetectors. The result is a practically aberration-free, fast-scanning high optical invariant two-photon microscopy (FASHIO-2PM) that enables calcium imaging from a large network composed of <16,000 neurons at 7.5 Hz froma9mm2 contiguous image plane, including more than 10 sensory-motor and higher-order areas of the cerebral cortex in awake mice. Network analysis based on single-cell activities revealed that the brain exhibits small-world rather than scale-free behavior. FASHIO-2PM will enable revealing biological dynamics by simultaneous monitoring of macroscopic activity and its composing elements. This paper is modified from Ota et al., Neuron 2021.

Conference Presentation

Citation Download Citation

Masanori Murayama "Fast, cell-resolution, wide field-of-view two-photon microscopy to reveal functional network architectures across multi-modal cortical areas", Proc. SPIE 11971, High-Speed Biomedical Imaging and Spectroscopy VII, 1197103 (2 March 2022); https://doi.org/10.1117/12.2612749

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