Glass integrated optofluidic sensor exploiting dielectrophoresis for label-free detection of bacterial viability

Konstantinos Akritidis; Mathilde Gardies; Gregory Grosa; Davide Bucci; Elise Ghibaudo

doi:10.1117/12.2607742

5 March 2022 Glass integrated optofluidic sensor exploiting dielectrophoresis for label-free detection of bacterial viability

Konstantinos Akritidis, Mathilde Gardies, Gregory Grosa, Davide Bucci, Elise Ghibaudo

Author Affiliations +

Proceedings Volume 12004, Integrated Optics: Devices, Materials, and Technologies XXVI; 120040M (2022) https://doi.org/10.1117/12.2607742
Event: SPIE OPTO, 2022, San Francisco, California, United States

Abstract

Water quality monitoring faces technological challenges such as rapid and in-situ measurements, using reusable, compact and easy-to-clean devices. Glass integrated photonics is an attractive solution: it exhibits a high sensitivity to absorption or interferometric measurements, and glass is chemically compatible with aqueous environments. An innovative idea for pollution detection is to assess the bacterial cellular viability as a global indicator for pollutant toxicity. This study proposes an original concept of integrated opto-fluidic sensor which sorts and quantifies the dead or alive bacteria in a liquid sample. To ensure a robust label-free detection, the cells discrimination is provided by a selective trapping of the bacteria exploiting dielectrophoretic effects. This avoids the use of a functionalization layer. The device comprises a photonic circuit made by silver-sodium ion-exchange on glass. The sensing area co-integrates a single-mode waveguide and aluminum electrodes designed to generate dielectrophoretic forces. Both waveguide and electrodes can be encapsulated inside a polydimethylsiloxane microfluidic channel for the flow of the bacterial suspensions. In this study, charged polystyrene beads (Sigma-Aldrich, CLB9) dispersed in deionized water have been used to model dead bacteria. We observed an intensity modulation of the guided light (up to 8% of the output power) at a wavelength of 1550 nm, by selectively controlling the beads trapping. We also correlated the beads collection by microscopy imaging.

Conference Presentation

Citation Download Citation

Konstantinos Akritidis, Mathilde Gardies, Gregory Grosa, Davide Bucci, and Elise Ghibaudo "Glass integrated optofluidic sensor exploiting dielectrophoresis for label-free detection of bacterial viability", Proc. SPIE 12004, Integrated Optics: Devices, Materials, and Technologies XXVI, 120040M (5 March 2022); https://doi.org/10.1117/12.2607742

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