Low-cost fabrication of PPIX liquid phantoms for use in fluorescence measurements

Valery Shupletsov; Margarita Mikenkina; Evgeny Zherebtsov; Viktor Dremin; Alexander Bykov; Elena Potapova; Andrey Dunaev; Igor Meglinski

doi:10.1117/12.2626389

29 April 2022 Low-cost fabrication of PPIX liquid phantoms for use in fluorescence measurements

Valery Shupletsov, Margarita Mikenkina, Evgeny Zherebtsov, Viktor Dremin, Alexander Bykov, Elena Potapova, Andrey Dunaev, Igor Meglinski

Author Affiliations +

Proceedings Volume 12192, Optical Technologies for Biology and Medicine; 121920U (2022) https://doi.org/10.1117/12.2626389
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.

Citation Download Citation

Valery Shupletsov, Margarita Mikenkina, Evgeny Zherebtsov, Viktor Dremin, Alexander Bykov, Elena Potapova, Andrey Dunaev, and Igor Meglinski "Low-cost fabrication of PPIX liquid phantoms for use in fluorescence measurements", Proc. SPIE 12192, Optical Technologies for Biology and Medicine, 121920U (29 April 2022); https://doi.org/10.1117/12.2626389

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