2p autofluorescence imaging endoscope for clinical observation of metabolic changes in cervical tissue

Berk Camli; Liam P. Andrus; Aditya Roy; Biswajit Mishra; Chris Xu; Irene Georgakoudi; Tomasz Tkaczyk; Adela Ben-Yakar

doi:10.1117/12.2650285

14 March 2023 2p autofluorescence imaging endoscope for clinical observation of metabolic changes in cervical tissue

Berk Camli, Liam P. Andrus, Aditya Roy, Biswajit Mishra, Chris Xu, Irene Georgakoudi, Tomasz Tkaczyk, Adela Ben-Yakar

Proceedings Volume 12356, Endoscopic Microscopy XVIII; 123560A (2023) https://doi.org/10.1117/12.2650285
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Imaging modalities capable of detecting functional changes over small areas can increase sensitivity and specificity of early cancer detection. Label-free imaging of metabolic activity at cellular level resolution over full thickness of cervix epithelium is possible with 2p imaging. However, low probability of 2p excitation and scattering nature of tissues limit autofluorescence levels in 2p imaging. We present a 2p autofluorescence imaging endoscope system for detection of metabolic changes in cervix in a clinical setting, with an increased collection efficiency in scattering media. Collection of autofluorescence signals is done with a multitude of high NA fibers arranged around a miniaturized excitation objective. By cleaving the collection fibers at a specific angle, we increase the directivity of the collection and the collection efficiency per fiber. The endoscope performs imaging at 775 nm, which is capable of exciting NAD(P)H and FAD molecules. Laser pulses of 100 fs duration are delivered to the sample with an air core photonic bandgap fiber. Fiber is scanned in spiral pattern via a piezo actuator tube. Scanning at different tissue depths is possible with the axial actuation of the endoscope via a linear stepper motor. Benchtop tests indicate that the endoscope system has lateral and axial resolutions of 0.65 μm and 4.33 μm, respectively. Fluorescence images of pollen cores are presented to demonstrate the imaging quality of the endoscope system.

Conference Presentation

Citation Download Citation

Berk Camli, Liam P. Andrus, Aditya Roy, Biswajit Mishra, Chris Xu, Irene Georgakoudi, Tomasz Tkaczyk, and Adela Ben-Yakar "2p autofluorescence imaging endoscope for clinical observation of metabolic changes in cervical tissue", Proc. SPIE 12356, Endoscopic Microscopy XVIII, 123560A (14 March 2023); https://doi.org/10.1117/12.2650285

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