Deriving clinical parameters for paired agent imaging in head and neck squamous cell carcinomas: efficient methodology for the measurement of plasma fluorescence

Sanjana Pannem; Cheng Wang; Divya Ravi; Sassan Hodge; Eunice Chen; Jonathan Elliott; Kenneth M. Tichauer; Keith Paulsen; Kimberley S. Samkoe

doi:10.1117/12.2649134

14 March 2023 Deriving clinical parameters for paired agent imaging in head and neck squamous cell carcinomas: efficient methodology for the measurement of plasma fluorescence

Sanjana Pannem, Cheng Wang, Divya Ravi, Sassan Hodge, Eunice Chen, Jonathan Elliott, Kenneth M. Tichauer, Keith Paulsen, Kimberley S. Samkoe

Author Affiliations +

Proceedings Volume 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX; 1236107 (2023) https://doi.org/10.1117/12.2649134
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Paired Agent Imaging (PAI) is a fluorescence imaging technique where a targeted probe is co-administered with an untargeted probe. PAI has been successfully demonstrated in a pre-clinical setting and its clinical translation is in progress. The tissue distribution and excretion of the two fluorescent dyes, ABY-029 and IRDY680LT, must display similar kinetics in order for the PAI model to hold. To study the excretion of the dyes, plasma studies need to be conducted to examine the presence of fluorescence in vivo over a select period of time. The current method of measuring plasma fluorescence involves centrifuging blood to isolate plasma and them measuring on a fluorometer which can be time consuming and inefficient. In this study, we examine multiple methods for visualizing and quantifying plasma fluorescence using blood and plasma phantoms at multiple concentrations. The phantom fluorescence was measured using the Pearl Imaging system and the Fluoromax-3. We have determined that imaging blood directly in a fluorescence imaging system provides the same information as plasma alone.

Conference Presentation

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Sanjana Pannem, Cheng Wang, Divya Ravi, Sassan Hodge, Eunice Chen, Jonathan Elliott, Kenneth M. Tichauer, Keith Paulsen, and Kimberley S. Samkoe "Deriving clinical parameters for paired agent imaging in head and neck squamous cell carcinomas: efficient methodology for the measurement of plasma fluorescence", Proc. SPIE 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX, 1236107 (14 March 2023); https://doi.org/10.1117/12.2649134

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