Photoacoustic microscopy using supercontinuum light for in vivo microscopic imaging of living tissue dynamics

Takeshi Hirasawa; Kazuyoshi Tachi; Manami Miyashita; Tomohiro Ishikawa; Keiichi Ito; Miya Ishihara

doi:10.1117/12.2652280

9 March 2023 Photoacoustic microscopy using supercontinuum light for in vivo microscopic imaging of living tissue dynamics

Takeshi Hirasawa, Kazuyoshi Tachi, Manami Miyashita, Tomohiro Ishikawa, Keiichi Ito, Miya Ishihara

Author Affiliations +

Proceedings Volume 12379, Photons Plus Ultrasound: Imaging and Sensing 2023; 123790Y (2023) https://doi.org/10.1117/12.2652280
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

To understand various phenomena that occur in living tissue, high-speed volume imaging technology that visualizes the dynamics of cells and molecules in living tissue is desired. For this purpose, we have developed a reflective MS-PAI with high excitation wavelength flexibility using a supercontinuum light source and bandpass filters, and a fast B-scan rate of over 100 fps. The ability of the MS-PAM for cell dynamics imaging was demonstrated by performing time-lapse volume imaging of contrast agent-stained cells flowing through blood vessels. We plan to combine the MS-PAM technology with functionalized contrast agents (imaging probes) to analyze dynamic phenomena.

Citation Download Citation

Takeshi Hirasawa, Kazuyoshi Tachi, Manami Miyashita, Tomohiro Ishikawa, Keiichi Ito, and Miya Ishihara "Photoacoustic microscopy using supercontinuum light for in vivo microscopic imaging of living tissue dynamics", Proc. SPIE 12379, Photons Plus Ultrasound: Imaging and Sensing 2023, 123790Y (9 March 2023); https://doi.org/10.1117/12.2652280

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available