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Optical resolution photoacoustic microscopy enables label-free imaging of structures containing a variety of endogenous chromophores. For fast image acquisition, we combine a galvo scanner with a fixed, acoustically focused detector, where the sample to be imaged is located slightly outside the acoustic focal plane at a distance where the whole field of view lies within the sensitive area of the acoustic detector. Errors arising due to the uneven sensitivity and the varying relation between arrival time and axial position are avoided by applying a model-based reconstruction to the received signals, using a calculated or measured three-dimensional point spread function.
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Guenther Paltauf, Robert Nuster, "Model-based reconstruction in optical-resolution photoacoustic microscopy," Proc. SPIE 12379, Photons Plus Ultrasound: Imaging and Sensing 2023, 123790Z (9 March 2023); https://doi.org/10.1117/12.2654237