Dual-wavelength low-cost high-resolution photoacoustic microscopy system for blood oxygenation imaging

Xiufeng Li; Bingxin Huang; Kylie Yeung; Victor T. C. Tsang; Claudia T. K. Lo; Terence T. W. Wong

doi:10.1117/12.2647870

9 March 2023 Dual-wavelength low-cost high-resolution photoacoustic microscopy system for blood oxygenation imaging

Xiufeng Li, Bingxin Huang, Kylie Yeung, Victor T. C. Tsang, Claudia T. K. Lo, Terence T. W. Wong

Proceedings Volume 12379, Photons Plus Ultrasound: Imaging and Sensing 2023; 1237918 (2023) https://doi.org/10.1117/12.2647870
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is a label-free and non-invasive technique for imaging blood vessel and hemoglobin oxygen saturation (sO₂) of living animals in vivo, providing functional information for disease diagnosis. However, most state-of-the-art OR-PAM systems require bulky and costly pulsed lasers, which hinders their wide applications in clinical settings. Here, a reflection-mode low-cost photoacoustic microscopy system using two laser diodes (LDs) was developed for in-vivo microvasculature and sO₂ imaging with a high resolution of ~6 μm. The sO2 measurement is validated in both blood phantom and in vivo animal experiments. The phantom study shows that our system has a strong linear relationship with the preset sO₂ (R ² = 0.96). The in-vivo experiment of mouse ear imaging demonstrated that our system can achieve high-resolution and high-quality imaging of microvasculature and sO₂. This technical advancement in cost reduction and superior imaging performance promotes the fast and wide applications of PAM in biomedical fields.

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Xiufeng Li, Bingxin Huang, Kylie Yeung, Victor T. C. Tsang, Claudia T. K. Lo, and Terence T. W. Wong "Dual-wavelength low-cost high-resolution photoacoustic microscopy system for blood oxygenation imaging", Proc. SPIE 12379, Photons Plus Ultrasound: Imaging and Sensing 2023, 1237918 (9 March 2023); https://doi.org/10.1117/12.2647870

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