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14 March 2023 Single cell-based rapid natural killer cell activity measurement by using lens-free shadow imaging technology (Conference Presentation)
Ahyeon Lee, Inha Lee, Myung-Hyun Nam, Hyun Sik Jun, Sungkyu Seo
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Proceedings Volume 12380, Biophotonics and Immune Responses XVIII; 123800H (2023) https://doi.org/10.1117/12.2648550
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We report a single cell-based rapid natural killer (NK) cell activity measurement technique that utilizes a newly developed activation stimulator cocktail (ASC) along with the lens-free shadow imaging platform. The platform, i.e., Cellytics_NK, consists of compact and low-cost optoelectronic components such as LED and CMOS image sensor, and the shadow images of photographed NK cells in a disposable cell chip are analyzed within 30 seconds with an automated algorithm. The shadow parameters such as PPD and WSM have been employed to induce a combined shadow parameter (CSP) which precisely measures and distinguishes quiescent and activated NK cells (p < 0.0001).
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Ahyeon Lee, Inha Lee, Myung-Hyun Nam, Hyun Sik Jun, and Sungkyu Seo "Single cell-based rapid natural killer cell activity measurement by using lens-free shadow imaging technology (Conference Presentation)", Proc. SPIE 12380, Biophotonics and Immune Responses XVIII, 123800H (14 March 2023); https://doi.org/10.1117/12.2648550
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KEYWORDS
Imaging technologies
Cancer
Blood
CMOS sensors
Light emitting diodes
Optoelectronic devices
Photography
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