Proof of concept for microfluidic spectral flow cytometry

Gillian C. McMahon; Kristen M. Wilding; Claire K. Sanders; Paul W. Fenimore; Douglas J. Perkins; Judith R. Mourant

doi:10.1117/12.2653140

15 March 2023 Proof of concept for microfluidic spectral flow cytometry

Gillian C. McMahon, Kristen M. Wilding, Claire K. Sanders, Paul W. Fenimore, Douglas J. Perkins, Judith R. Mourant

Proceedings Volume 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; 123830K (2023) https://doi.org/10.1117/12.2653140
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Results are presented from an experimental proof of concept for a full-spectrum microfluidic flow cytometer. Existing flow cytometers operate on photomultiplier tubes (PMTs) and are restricted in the number of available fluorophores, with newer systems costing $250k to $500k. Current EMCCD technology paired with microfluidics promises to offer superior channel decomposition while allowing a significant reduction in price. A device was constructed from polydimethysiloxane (PDMS) with fiber optics for sample excitation and fluorescence collection, and with an 80 micron channel for sample flow. In the first experiment, we captured spectra of individual fluorescently labeled polystyrene beads and demonstrated classification of beads according to their labeling fluorophore, as well as characterization of anomalous beads. In the second experiment, two stoichiometric mixtures of six dyes were created with the successful decomposition of the mixtures into components, using reference spectra acquired on the system. We anticipate the capability of full spectra to enable the extension to twenty-one fluorophores with the microfluidic design creating a robust system with a total cost of under $50,000.

Citation Download Citation

Gillian C. McMahon, Kristen M. Wilding, Claire K. Sanders, Paul W. Fenimore, Douglas J. Perkins, and Judith R. Mourant "Proof of concept for microfluidic spectral flow cytometry", Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830K (15 March 2023); https://doi.org/10.1117/12.2653140

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